What is the best In Vitro assay? Alamar Blue, Presto Blue.
The WST-1 Cell Proliferation Colorimetric Assay Kit provides a fast and sensitive way for quantification of cell proliferation and viability. Cell proliferation causes the increase in the amount of formazan dye formed that can be quantified by measuring the absorbance of the dye solution at 440 nm using microtiter plate reader. The kit can be used for the analysis of cell proliferation in.
The detection sensitivity of CCK-8 is higher than the other tetrazolium salts such as MTT, XTT, MTS or WST-1. Cell viability detection mechanism with CCK-8 Procedure. Cell Counting Kit-8 requires 3 simple steps. The handling time of Cell Counting Kit-8 is the shortest among its competitors. Only 15 minutes of handling time is needed for Cell Counting Kit-8, whereas longer handling time is.
The amount of formazan product is directly proportional to the enzymatic activity of dehydrogenases and the number of living cells. With its high sensitivity and low cytotoxicity, Cyto-X outperforms traditional assays using other tetrazolium salts such as MTT, XTT, MTS or WST-1.
The CyQUANT XTT Cell Viability Assay is a complete and optimized kit for the detection of mammalian cell viability. The assay kit includes XTT Reagent (2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide) and Electron Coupling Reagent. The XTT reagent, which is a tetrazolium-based compound, is sensitive to cellular redox potential and in the presence of actively respiring.
MTT can readily penetrates the cells forming an insoluble formazan whereas WST cannot penetrates the cells which requires extracellularly reduced by the plasma membrane. However, MTT required an.
Hence while performing cell viability with different tetarazolium dyes they produce different results depending on whether they are reduced intracellularly (MTT, MTS) or extracellularly (WST-1).
The kit utilizes a highly water-soluble tetrazolium salt, WST-8, which produces a water-soluble formazan dye upon reduction in the presence of an electron mediator. The amount of the formazan generated by dehydrogenases is directly in proportion to the numbers of living cells. The detection sensitivity by CCK-8 is higher than other tetrazolium salts such as MTT, XTT, MTS or WST-1.